Fibrotic effect of monoculture, conditioned media and coculture of fibroblast NIH3T3 cells on collagen secretion and mRNA TGF-beta expressions

Fibrosis is common profound in diabetes condition, they characterized by accumulation of extracellular matrix tissue then cause injury. The study aim to investigate effect monoculture, conditioned media, and coculture NIH3T3 cells LPS-high glucose media conditions on mRNA TGF-B collagen secretion. fibroblast were cultured with various culture type there mono NIH3T3, from RAW264.7 (ratio 1:1 1:2) normal (media containing 4500 mikro g/mL glucose) HG-LPS LPS 0.25 D-glucose 5400 g/mL). Our shows that secretion monoculture inflammatory macrophages are not statistically different. On other hand, the expression relative ratio RAW 264.7 NIH 3T3 cell a highest compared models (p kurang dari 0.05). conclude (1:1) high appropriate for fibrosis vitro models.